Journal: Methods (San Diego, Calif.)

Article Title: In Vitro Selection of Proteins with Desired Characteristics Using mRNA-display

doi: 10.1016/j.ymeth.2012.11.004

Figure Lengend Snippet:

Article Snippet: Assemble the following 80 µl reaction mixture: Component Volume per reaction (µl) Final concentration Digested cDNA from Step 25 50 — T4 ligation buffer, 10× (NEB) 8 1× Digested/purified EcoRI-HF left cassette (see Oligos Table) X — Digested/purified HindIII right cassette (see Oligos Table) X — T4 DNA ligase (6 Weiss U/µl) 8 0.6 U/µl Nuclease-free water To a final concentration of 80 µl Open in a separate window Mix gently and incubate the ligation mixture at 16 °C for 20 h. Size separation and PCR amplification of the cDNA library .

Techniques: Concentration Assay

Journal: Methods (San Diego, Calif.)

Article Title: In Vitro Selection of Proteins with Desired Characteristics Using mRNA-display

doi: 10.1016/j.ymeth.2012.11.004

Figure Lengend Snippet:

Article Snippet: Assemble the following 80 µl reaction mixture: Component Volume per reaction (µl) Final concentration Digested cDNA from Step 25 50 — T4 ligation buffer, 10× (NEB) 8 1× Digested/purified EcoRI-HF left cassette (see Oligos Table) X — Digested/purified HindIII right cassette (see Oligos Table) X — T4 DNA ligase (6 Weiss U/µl) 8 0.6 U/µl Nuclease-free water To a final concentration of 80 µl Open in a separate window Mix gently and incubate the ligation mixture at 16 °C for 20 h. Size separation and PCR amplification of the cDNA library .

Techniques: Concentration Assay, Ligation

Journal: Methods in enzymology

Article Title: Quantitative Measurement of PROTAC Intracellular Accumulation

doi: 10.1016/bs.mie.2022.11.001

Figure Lengend Snippet: 2.2. KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cell Lines E. coli derived from the BLT5403 strain T7 Select 10–3 strain Novagen 70548–3 Chemicals, Peptides, and Recombinant Proteins SEA BLOCK Blocking Buffer Thermo Scientific 37527 nLuc-BTK construct (full length) Eurofins Customized EcoRI-HF NEB R3101S HindIII NEB R0104S T4 DNA Ligase NEB M0202T T4 DNA Ligase Reaction Buffer NEB B0202S Streptavidin-coated magnetic beads Thermo Scientific 88816 biotinylated small molecule ligands Critical Commercial Assays MinElute PCR Purification Kit Qiagen 28004 T7Select ® Packaging Kit Novagen 70014–3 Open in a separate window 2.2.

Techniques: Derivative Assay, Recombinant, Blocking Assay, Construct, Magnetic Beads, Purification